Extraction Using Magnetic Beads
Magnetic separation:
- DNA selectively binds to an appropriately-coated bead surface, leaving contaminants in solution.
- Advantage of using this: DNA can be isolated directly from a crude sample, with minimal processing.
- Same protocol for different types of samples.
- Less than 15 mins for the protocol to be completed - does not depend on centrifuge too.
- After binding DNA, an external magnetic field attracts the beads to the outer edge of the containing tube, immobilizing them. While the beads are immobilized, the bead-bound DNA is retained during the washing steps. Adding elution buffer, and removing the magnetic field then releases the DNA as a purified sample, ready for quantitation and analysis.
Im not sure about ordering reagents for the Magnetic Beads Extraction